Hotspot

trnP-UGG-psaJ

LSC · 73941-74398 · 458 bp

Back to Asclepias

Pi 0.0134

MarkerSeek score 65.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 6
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TCGTTACTGCTCAACGTAGC	GCGTACTTAGTACTGGTGCC	50.0	55.0	58.0	58.1	600-610 bp	1.0	81.2
trnP-UGG-psaJ_p2	ACCCGTGACATTTTGTACCC	GCGTACTTAGTACTGGTGCC	50.0	55.0	58.1	58.1	552-562 bp	1.0	80.8
trnP-UGG-psaJ_p3	CCCGTGACATTTTGTACCCA	GCGTACTTAGTACTGGTGCC	50.0	55.0	58.1	58.1	551-561 bp	1.0	80.8
trnP-UGG-psaJ_p4	ACAGGATTTGAACCCGTGAC	GCGTACTTAGTACTGGTGCC	50.0	55.0	58.1	58.1	563-573 bp	1.0	80.7
trnP-UGG-psaJ_p5	CAGGATTTGAACCCGTGACA	GCGTACTTAGTACTGGTGCC	50.0	55.0	58.1	58.1	562-572 bp	1.0	80.7