Hotspot

ycf4-cemA

LSC · 67991-68712 · 722 bp

Back to Asclepias

Pi 0.016

MarkerSeek score 47.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 7
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTCGCATATTCCTCCGATT	CTTGTGTAGACGTGTCTCCG	50.0	55.0	57.9	58.0	886-1175 bp	1.0	86.1
ycf4-cemA_p2	TGGTGCACAATTTCCTGGAA	CTTGTGTAGACGTGTCTCCG	45.0	55.0	57.9	58.0	985-1274 bp	1.0	85.8
ycf4-cemA_p3	GGAGCCATTCCCTTAACTCG	CTTGTGTAGACGTGTCTCCG	55.0	55.0	58.0	58.0	778-1067 bp	1.0	85.6
ycf4-cemA_p4	GGTATTTATGCCCGTCGTGT	CTTGTGTAGACGTGTCTCCG	50.0	55.0	58.1	58.0	1112 bp	0.5	59.3
ycf4-cemA_p5	AATCGTCGCATATTCCTCCG	CTTGTGTAGACGTGTCTCCG	50.0	55.0	57.9	58.0	1178 bp	0.5	59.2