Hotspot

rbcL-accD

LSC · 63390-64274 · 885 bp

Back to Asclepias

Pi 0.0132

MarkerSeek score 65.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 11
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CAATAGGACCAAGACTGCCC	55.0	55.0	58.0	58.0	1035-1069 bp	1.0	84.2
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	ATAGGACCAAGACTGCCCAT	55.0	50.0	58.0	58.1	1033-1067 bp	1.0	83.7
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	CAATAGGACCAAGACTGCCC	50.0	55.0	57.9	58.0	1005-1039 bp	1.0	83.6
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	GACCAAGACTGCCCATTGAT	55.0	50.0	58.0	57.9	1029-1063 bp	1.0	83.4
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	ATAGGACCAAGACTGCCCAT	50.0	50.0	57.9	58.1	1003-1037 bp	1.0	83.1