Hotspot

ndhC-trnV-UAC

LSC · 56913-58188 · 1276 bp

Back to Asclepias

Pi 0.0204
MarkerSeek score 63.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1276
variable_sites
24
pi
0.0204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGTTAATCGGGGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1321-1421 bp 1.0 82.8
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1307-1407 bp 1.0 82.7
ndhC-trnV-UAC_p3 GTCCTTTGTTAATCGGGGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1320-1420 bp 1.0 80.1
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1306-1406 bp 1.0 80.0
ndhC-trnV-UAC_p5 TGGTCCTTTGTTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1323-1423 bp 1.0 79.8