Hotspot

rps2-rpoC2

LSC · 16581-21548 · 4968 bp

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Pi 0.0173
MarkerSeek score 40.90
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
4968
variable_sites
46
pi
0.0173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.54
markerseek_score
40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 TGAATTTTTGTGGTACTCTTGTCA ATCCCTCTGTCGAACACATA 33.3 45.0 57.0 55.7 170 bp 1.0 47.1
rps2-rpoC2_p2 TGAATTTTTGTGGTACTCTTGTCA TCCCTCTGTCGAACACATAT 33.3 45.0 57.0 55.7 169 bp 1.0 47.1
rps2-rpoC2_p3 TGAATTTTTGTGGTACTCTTGTCA ATATCCCTCTGTCGAACACA 33.3 45.0 57.0 55.7 172 bp 1.0 47.0
rps2-rpoC2_p4 TGAATTTTTGTGGTACTCTTGTCA TATCCCTCTGTCGAACACAT 33.3 45.0 57.0 55.7 171 bp 1.0 47.0
rps2-rpoC2_p5 TGAATTTTTGTGGTACTCTTGTCA AATATCCCTCTGTCGAACACA 33.3 42.9 57.0 56.5 173 bp 1.0 46.9