Hotspot

atpH-atpI

LSC · 13736-14895 · 1160 bp

Back to Asclepias

Pi 0.0147

MarkerSeek score 67.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 17
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTCTACAGCTTGACCTGCAG	CGCGGCTTATATCGGTGAAT	55.0	50.0	58.0	58.3	1311-1319 bp	1.0	78.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGCGGCTTATATCGGTGAAT	45.0	50.0	58.0	58.3	1232-1240 bp	1.0	78.6
atpH-atpI_p3	CTCTACAGCTTGACCTGCAG	CGGTGAATCCATGGAAGGTC	55.0	55.0	58.0	58.3	1299-1307 bp	1.0	78.5
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CGGTGAATCCATGGAAGGTC	45.0	55.0	58.0	58.3	1220-1228 bp	1.0	78.3
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	CGCGGCTTATATCGGTGAAT	45.0	50.0	58.2	58.3	1233-1241 bp	1.0	77.4