Hotspot

trnQ-UUG-psbK

LSC · 7521-8096 · 576 bp

Back to Asclepias

Pi 0.046
MarkerSeek score 75.90
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
576
variable_sites
24
pi
0.046
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 AAAACCCGTTGCCTTACCAC AAAAAGCGTAGGCCTCGG 50.0 55.6 59.0 57.7 653-707 bp 1.0 60.7
trnQ-UUG-psbK_p2 CAAAACCCGTTGCCTTACCA AAAAAGCGTAGGCCTCGG 50.0 55.6 59.0 57.7 654-708 bp 1.0 60.7
trnQ-UUG-psbK_p3 CCCAAAACCCGTTGCCTTA AAAAAGCGTAGGCCTCGG 52.6 55.6 58.3 57.7 656-710 bp 1.0 58.3
trnQ-UUG-psbK_p4 AAACCCGTTGCCTTACCAC AAAAAGCGTAGGCCTCGG 52.6 55.6 58.3 57.7 652-706 bp 1.0 58.2
trnQ-UUG-psbK_p5 AAAACCCGTTGCCTTACCA AAAAAGCGTAGGCCTCGG 47.4 55.6 57.5 57.7 653-707 bp 1.0 56.3