Hotspot

cemA

LSC · 64496-65239 · 744 bp

Back to Ascarina

Pi 0.00672

MarkerSeek score 50.50

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 5
pi: 0.00672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AGTCGACGAATGAAACAGGT	CCTGGACGGGTATAAAAGAGT	45.0	47.6	57.2	56.8	867 bp	1.0	56.5
cemA_p2	AGTCGACGAATGAAACAGGT	CCTGGACGGGTATAAAAGAGTG	45.0	50.0	57.2	58.2	867 bp	1.0	56.3
cemA_p3	AGTCGACGAATGAAACAGGTT	CCTGGACGGGTATAAAAGAGT	42.9	47.6	57.9	56.8	867 bp	1.0	54.1
cemA_p4	AGTCGACGAATGAAACAGGTT	CCTGGACGGGTATAAAAGAGTG	42.9	50.0	57.9	58.2	867 bp	1.0	53.9
cemA_p5	GAGTCGACGAATGAAACAGGT	CCTGGACGGGTATAAAAGAGT	47.6	47.6	58.3	56.8	868 bp	1.0	52.4