Hotspot

trnL-UAG-rpl32

SSC · 127631-128697 · 1067 bp

Back to Ascarina

Pi 0.0122

MarkerSeek score 63.40

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 1067
variable_sites: 13
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGCTGCGATAAAAGCGTTTT	50.0	45.0	58.0	58.0	1169-1189 bp	1.0	80.6
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGCTGCGATAAAAGCGTTTT	50.0	45.0	58.0	58.0	1170-1190 bp	1.0	80.6
trnL-UAG-rpl32_p3	TCCTAAGAGCAGCGTGTCTA	GGCTGCGATAAAAGCGTTTT	50.0	45.0	57.9	58.0	1179-1199 bp	1.0	79.7
trnL-UAG-rpl32_p4	TTAGAATATGCCGCCACTCG	GGCTGCGATAAAAGCGTTTT	50.0	45.0	57.9	58.0	1228-1248 bp	1.0	79.5
trnL-UAG-rpl32_p5	CTAAGAGCAGCGTGTCTACC	GGCTGCGATAAAAGCGTTTT	55.0	45.0	57.8	58.0	1177-1197 bp	1.0	79.1