Hotspot

petG-trnW-CCA

LSC · 69410-69553 · 144 bp

Back to Ascarina

Pi 0.0147

MarkerSeek score 65.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 2
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GCGATCAGTTGGACCTTTGA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.2	58.0	209-217 bp	1.0	81.9
petG-trnW-CCA_p2	GCGATCAGTTGGACCTTTGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.2	57.9	195-203 bp	1.0	81.8
petG-trnW-CCA_p3	GCGATCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.2	58.0	231-239 bp	1.0	81.7
petG-trnW-CCA_p4	GCGATCAGTTGGACCTTTGA	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.2	57.6	190-198 bp	1.0	79.6
petG-trnW-CCA_p5	GCGATCAGTTGGACCTTTGA	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	58.2	57.7	229-237 bp	1.0	79.1