Hotspot

ndhC-trnV-UAC

LSC · 52809-54427 · 1619 bp

Back to Ascarina

Pi 0.00755

MarkerSeek score 64.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1619
variable_sites: 12
pi: 0.00755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1729-1750 bp	1.0	79.2
ndhC-trnV-UAC_p2	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1742-1763 bp	1.0	76.6
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1728-1749 bp	1.0	76.5
ndhC-trnV-UAC_p4	TCCGGAAATGAGAAATGCCA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.5	57.8	1718-1739 bp	1.0	76.3
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.4	57.8	1746-1767 bp	1.0	75.5