Hotspot

cemA

LSC · 71900-72589 · 690 bp

Pi 0.00145

MarkerSeek score 52.40

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 2
pi: 0.00145
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	ACGGAGTCGATGAATGGAGT	CCTGCACGGATGTCAGATTT	50.0	50.0	58.5	58.3	829-831 bp	1.0	74.0
cemA_p2	ACGGAGTCGATGAATGGAGT	ATCCGCGGATCAAATGAGTT	50.0	45.0	58.5	57.7	764-766 bp	1.0	73.5
cemA_p3	CCTTGTTGTCGAACTCATGC	CCTGCACGGATGTCAGATTT	50.0	50.0	57.4	58.3	870-872 bp	1.0	73.4
cemA_p4	ACGGAGTCGATGAATGGAGT	CCGCGGATCAAATGAGTTCT	50.0	50.0	58.5	58.3	762-764 bp	1.0	73.4
cemA_p5	ACGGAGTCGATGAATGGAGT	TCCGCGGATCAAATGAGTTC	50.0	50.0	58.5	58.3	763-765 bp	1.0	73.3