Hotspot

petA-psbJ

LSC · 73762-74830 · 1069 bp

Back to Asarum

Pi 0.00581
MarkerSeek score 55.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1069
variable_sites
15
pi
0.00581
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCATCTGTTACTCTGGCACA CTGTGATTGGTTCAGTGGGT 50.0 50.0 57.9 57.7 1217-1218 bp 1.0 78.0
petA-psbJ_p2 TTGGCATCTGTTACTCTGGC CTGTGATTGGTTCAGTGGGT 50.0 50.0 57.9 57.7 1220-1221 bp 1.0 77.9
petA-psbJ_p3 ACGTGTCCAAGGTTTGTTGT CTGTGATTGGTTCAGTGGGT 45.0 50.0 58.2 57.7 1245-1246 bp 1.0 77.4
petA-psbJ_p4 GCATCTGTTACTCTGGCACA CCTGTGATTGGTTCAGTGGG 50.0 55.0 57.9 58.5 1218-1219 bp 1.0 76.6
petA-psbJ_p5 TTGGCATCTGTTACTCTGGC CCTGTGATTGGTTCAGTGGG 50.0 55.0 57.9 58.5 1221-1222 bp 1.0 76.4