Hotspot

psbA-trnH-GUG

LSC · 33983-35693 · 1711 bp

Back to Asarum

Pi 0.0107
MarkerSeek score 64.70
Primer available yes
Label psbA-trnH-GUG

Feature Metadata

label_name
psbA-trnH-GUG
feature_type
igs
region
LSC
length_bp
1711
variable_sites
35
pi
0.0107
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnH-GUG_p1 AACCGTGCAAACCTTGGTAT GATCCACTTGGCTACATCCG 45.0 55.0 58.0 58.1 1785-1903 bp 1.0 84.4
psbA-trnH-GUG_p2 AACCGTGCAAACCTTGGTAT CAATCCACTGCCTTGATCCA 45.0 50.0 58.0 57.9 1799-1917 bp 1.0 84.4
psbA-trnH-GUG_p3 AACCGTGCAAACCTTGGTAT AATCCACTGCCTTGATCCAC 45.0 50.0 58.0 57.9 1798-1916 bp 1.0 84.4
psbA-trnH-GUG_p4 AACCGTGCAAACCTTGGTAT ACAATCCACTGCCTTGATCC 45.0 50.0 58.0 57.9 1800-1918 bp 1.0 84.4
psbA-trnH-GUG_p5 CCTCTAGACCTAGCTGCTGT GATCCACTTGGCTACATCCG 55.0 55.0 57.9 58.1 1731-1849 bp 1.0 84.0