Hotspot

rps16-trnK-UUU

LSC · 29001-29899 · 899 bp

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Pi 0
MarkerSeek score 51.70
Primer available yes
Label rps16-trnK-UUU

Feature Metadata

label_name
rps16-trnK-UUU
feature_type
igs
region
LSC
length_bp
899
variable_sites
0
pi
0
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnK-UUU_p1 TCTTGGAAAGGGTGCTCAAC AAAGCCGAGTACTCTACCGT 50.0 50.0 58.0 58.2 999-1003 bp 1.0 78.8
rps16-trnK-UUU_p2 TCTTGGAAAGGGTGCTCAAC AAGCCGAGTACTCTACCGTT 50.0 50.0 58.0 58.2 998-1002 bp 1.0 78.8
rps16-trnK-UUU_p3 GGTGCTCAACCTACAGGAAC AAAGCCGAGTACTCTACCGT 55.0 50.0 58.2 58.2 989-993 bp 1.0 77.5
rps16-trnK-UUU_p4 GGTGCTCAACCTACAGGAAC AAGCCGAGTACTCTACCGTT 55.0 50.0 58.2 58.2 988-992 bp 1.0 77.5
rps16-trnK-UUU_p5 TCTTGGAAAGGGTGCTCAAC TACCGTTGAGTTAGCAACCC 50.0 50.0 58.0 57.5 985-989 bp 1.0 76.5