Hotspot

atpH-atpF

LSC · 19190-19704 · 515 bp

Back to Asarum

Pi 0.0157
MarkerSeek score 65.30
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
LSC
length_bp
515
variable_sites
15
pi
0.0157
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 AATTTACGGACTGGTCGTGG ATTAAACCCGAAACTCCCGG 50.0 50.0 57.9 57.9 639-1144 bp 1.0 82.0
atpH-atpF_p2 AATTTACGGACTGGTCGTGG GGCCAGTGACCTAAGGAAAC 50.0 55.0 57.9 58.2 614-1119 bp 1.0 81.6
atpH-atpF_p3 TCGTGGCATTAGCGCTTTTA ATTAAACCCGAAACTCCCGG 45.0 50.0 58.3 57.9 625-1130 bp 1.0 80.6
atpH-atpF_p4 AATTTACGGACTGGTCGTGG ATGGCCAGTGACCTAAGGAA 50.0 50.0 57.9 58.3 616-1121 bp 1.0 80.4
atpH-atpF_p5 TCGTGGCATTAGCGCTTTTA GGCCAGTGACCTAAGGAAAC 45.0 55.0 58.3 58.2 600-1105 bp 1.0 80.2