Hotspot

petA-psbJ

LSC · 61930-62852 · 923 bp

Back to Arundo

Pi 0.0311
MarkerSeek score 70.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
923
variable_sites
43
pi
0.0311
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC ACGGTTCGTATTCTGGATTGG 50.0 47.6 57.9 58.1 1047-1060 bp 1.0 75.5
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC GGTTCGTATTCTGGATTGGGT 50.0 47.6 57.9 57.7 1045-1058 bp 1.0 74.1
petA-psbJ_p3 GGCCTTTTGTTCTTCTTCGC CGGTTCGTATTCTGGATTGG 50.0 50.0 57.9 56.3 1046-1059 bp 1.0 70.8
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT ACGGTTCGTATTCTGGATTGG 50.0 47.6 58.7 58.1 1054-1067 bp 1.0 70.4
petA-psbJ_p5 GGCCTTTTGTTCTTCTTCGC CGGTTCGTATTCTGGATTGGG 50.0 52.4 57.9 58.8 1046-1059 bp 1.0 70.0