Hotspot

rpl23-psaI

LSC · 58318-58641 · 324 bp

Back to Arundo

Pi 0.0576
MarkerSeek score 74.30
Primer available yes
Label rpl23-psaI

Feature Metadata

label_name
rpl23-psaI
feature_type
igs
region
LSC
length_bp
324
variable_sites
25
pi
0.0576
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-psaI_p1 AAGCCGGTAGATACCATCGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 941-1003 bp 1.0 87.1
rpl23-psaI_p2 CAAGCCGGTAGATACCATCG CCATTGCAATTGCCGGAAAT 55.0 45.0 57.9 58.0 942-1004 bp 1.0 86.6
rpl23-psaI_p3 AAGCCGGTAGATACCATCGA CCAAGATAACTGGAAGCCCC 50.0 55.0 58.0 57.9 1474-1541 bp 1.0 86.3
rpl23-psaI_p4 AAGCCGGTAGATACCATCGA CGCGATTCCGTAGAAAGACA 50.0 50.0 58.0 58.1 1556-1623 bp 1.0 85.9
rpl23-psaI_p5 AAGCCGGTAGATACCATCGA GGAGCTAATGAATAGGCCCG 50.0 55.0 58.0 57.9 1574-1641 bp 1.0 85.8