Hotspot

trnR-UCU-rps14

LSC · 37661-38046 · 386 bp

Back to Arundo

Pi 0.0432
MarkerSeek score 62.20
Primer available yes
Label trnR-UCU-rps14

Feature Metadata

label_name
trnR-UCU-rps14
feature_type
igs
region
LSC
length_bp
386
variable_sites
25
pi
0.0432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-rps14_p1 AGAAGACCTCTGTCCTATCCA ATGCATGTTTGTTACCGGGT 47.6 45.0 56.9 58.1 463-464 bp 1.0 67.2
trnR-UCU-rps14_p2 AGAAGACCTCTGTCCTATCCA TCCGGACACATACTTCGAGA 47.6 50.0 56.9 57.9 491-492 bp 1.0 66.7
trnR-UCU-rps14_p3 AGGTTTAGAAGACCTCTGTCCT ATGCATGTTTGTTACCGGGT 45.5 45.0 57.9 58.1 469-470 bp 1.0 66.5
trnR-UCU-rps14_p4 AGAAGACCTCTGTCCTATCCA TTACCGGGTGCAACAAGATC 47.6 50.0 56.9 58.2 452-453 bp 1.0 66.5
trnR-UCU-rps14_p5 AGGTTTAGAAGACCTCTGTCCT TCCGGACACATACTTCGAGA 45.5 50.0 57.9 57.9 497-498 bp 1.0 66.0