Hotspot

trnY-GUA-trnD-GUC

LSC · 16327-16679 · 353 bp

Back to Arundo

Pi 0.0409
MarkerSeek score 76.30
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
353
variable_sites
21
pi
0.0409
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 430-445 bp 1.0 80.9
trnY-GUA-trnD-GUC_p2 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.6 58.5 429-444 bp 1.0 80.7
trnY-GUA-trnD-GUC_p3 TGGGGACGGACTGTAAATTC CGGTGCTCTGACCAATTGAA 50.0 50.0 57.2 58.5 431-446 bp 1.0 77.5
trnY-GUA-trnD-GUC_p4 GGGGACGGACTGTAAATTCG GGTGCTCTGACCAATTGAAC 55.0 50.0 58.4 57.0 429-444 bp 1.0 76.7
trnY-GUA-trnD-GUC_p5 GGGACGGACTGTAAATTCGT GGTGCTCTGACCAATTGAAC 50.0 50.0 57.6 57.0 428-443 bp 1.0 76.6