Hotspot

trnT-GGU-trnE-UUC

LSC · 15597-16125 · 529 bp

Back to Arundo

Pi 0.0196
MarkerSeek score 65.00
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
529
variable_sites
15
pi
0.0196
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGCCCTTTTAACTCAGTGGT GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.7 58.3 563-652 bp 1.0 73.0
trnT-GGU-trnE-UUC_p2 AGCCCTTTTAACTCAGTGGT TGTCCTGAACCACTAGACGA 45.0 50.0 56.7 57.7 542-631 bp 1.0 73.0
trnT-GGU-trnE-UUC_p3 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.6 58.3 526-615 bp 1.0 73.0
trnT-GGU-trnE-UUC_p4 AGGCATAAGTCATCGGTTCA TGTCCTGAACCACTAGACGA 45.0 50.0 56.6 57.7 505-594 bp 1.0 72.9
trnT-GGU-trnE-UUC_p5 TGGTAAGGCATAAGTCATCGG GCTGCCTCCTTGAAAGAGAG 47.6 55.0 57.5 58.3 531-620 bp 1.0 72.2