Hotspot

trnS-CGA-trnT-GGU

LSC · 14005-15522 · 1518 bp

Back to Arundo

Pi 0.0299
MarkerSeek score 69.60
Primer available yes
Label trnS-CGA-trnT-GGU

Feature Metadata

label_name
trnS-CGA-trnT-GGU
feature_type
igs
region
LSC
length_bp
1518
variable_sites
68
pi
0.0299
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-CGA-trnT-GGU_p1 ACGAATCACACTTTTACCACT ACCACTGAGTTAAAAGGGCT 38.1 45.0 55.5 56.7 1570-2013 bp 1.0 45.7
trnS-CGA-trnT-GGU_p2 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 1637-2080 bp 1.0 45.5
trnS-CGA-trnT-GGU_p3 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1607-2050 bp 1.0 45.4
trnS-CGA-trnT-GGU_p4 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1603-2046 bp 1.0 44.7
trnS-CGA-trnT-GGU_p5 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1604-2047 bp 1.0 44.7