Hotspot

psbK-psbI

LSC · 7444-7823 · 380 bp

Back to Arundo

Pi 0.0219
MarkerSeek score 63.70
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
380
variable_sites
12
pi
0.0219
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TCCAAAGATAAAGAGGGAAACA 50.0 36.4 57.9 55.0 480-513 bp 1.0 45.9
psbK-psbI_p2 TTAGCGTTTGTTTGGCAAGC TCCAAAGATAAAGAGGGAAACA 45.0 36.4 58.2 55.0 489-522 bp 1.0 45.9
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TCCAAAGATAAAGAGGGAAACAA 50.0 34.8 57.9 55.7 480-513 bp 1.0 45.9
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT TCCAAAGATAAAGAGGGAAACA 45.0 36.4 58.3 55.0 477-510 bp 1.0 45.9
psbK-psbI_p5 TTGGCAAGCTGCTGTAAGTT TCCAAAGATAAAGAGGGAAACA 45.0 36.4 58.3 55.0 478-511 bp 1.0 45.9