Hotspot

rps16-trnQ-UUG

LSC · 4749-6829 · 2081 bp

Back to Arundo

Pi 0.0181
MarkerSeek score 63.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2081
variable_sites
56
pi
0.0181
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCGAACATCAATTGCAACGA GAGGTTCGAATCCTTCCGTC 40.0 55.0 56.4 58.1 2126-2366 bp 1.0 72.2
rps16-trnQ-UUG_p2 TCGAACATCAATTGCAACGA CTCGGAGGTTCGAATCCTTC 40.0 55.0 56.4 57.8 2130-2370 bp 1.0 71.1
rps16-trnQ-UUG_p3 CGAGATCGAACATCAATTGCA GAGGTTCGAATCCTTCCGTC 42.9 55.0 57.1 58.1 2131-2371 bp 1.0 69.7
rps16-trnQ-UUG_p4 TCGAACATCAATTGCAACGA TACTCGGAGGTTCGAATCCT 40.0 50.0 56.4 57.6 2132-2372 bp 1.0 69.3
rps16-trnQ-UUG_p5 TCGAACATCAATTGCAACGA ACTCGGAGGTTCGAATCCTT 40.0 50.0 56.4 58.4 2131-2371 bp 1.0 69.2