Hotspot

rbcL-psaI

LSC · 58545-59504 · 960 bp

Back to Arundinella

Pi 0.0103

MarkerSeek score 58.70

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 960
variable_sites: 31
pi: 0.0103
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCAAATTTGAGTTCGCCCCG	GTCATTGCAATTGCCGGAAA	50.0	45.0	59.1	57.9	1052-1113 bp	1.0	74.8
rbcL-psaI_p2	ATCAAATTTGAGTTCGCCCC	GTCATTGCAATTGCCGGAAA	45.0	45.0	56.7	57.9	1053-1114 bp	1.0	73.2
rbcL-psaI_p3	CAAATTTGAGTTCGCCCCG	GTCATTGCAATTGCCGGAAA	52.6	45.0	57.6	57.9	1051-1112 bp	1.0	72.6
rbcL-psaI_p4	TCAAATTTGAGTTCGCCCCG	GAAGTCATTGCAATTGCCGG	50.0	50.0	59.1	58.4	1055-1116 bp	1.0	72.5
rbcL-psaI_p5	TCAAATTTGAGTTCGCCCCG	GGAAGTCATTGCAATTGCCG	50.0	50.0	59.1	58.4	1056-1117 bp	1.0	72.5