Hotspot

atpB-rbcL

LSC · 56306-57113 · 808 bp

Back to Arundinella

Pi 0.00876
MarkerSeek score 56.00
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
808
variable_sites
19
pi
0.00876
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AGTAGTAGGATTGGTTCTCA AGTTTCTGTTTGTGGTGACA 40.0 40.0 52.3 55.3 834-852 bp 1.0 44.5
atpB-rbcL_p2 AGTAGTAGGATTGGTTCTCAT AGTTTCTGTTTGTGGTGACA 38.1 40.0 53.1 55.3 834-852 bp 1.0 44.5
atpB-rbcL_p3 AGTAGTAGGATTGGTTCTCA AGTTTCTGTTTGTGGTGACAT 40.0 38.1 52.3 55.9 834-852 bp 1.0 44.5
atpB-rbcL_p4 AGTAGTAGGATTGGTTCTCA TGCTTTAGTTTCTGTTTGTGGTG 40.0 39.1 52.3 58.0 840-858 bp 1.0 44.4
atpB-rbcL_p5 AGTAGTAGGATTGGTTCTCA ACACCAGGTTTAAATCCAACAC 40.0 40.9 52.3 57.1 863-881 bp 1.0 44.3