Hotspot

trnR-UCU-trnfM-CAU

LSC · 38531-38697 · 167 bp

Back to Arundinella

Pi 0.0416

MarkerSeek score 64.00

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 19
pi: 0.0416
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	TTGGGGTTACGGGTTCGAT	47.6	52.6	56.9	58.6	222 bp	1.0	57.4
trnR-UCU-trnfM-CAU_p2	AGGTTTAGAAGACCTCTGTCCT	TTGGGGTTACGGGTTCGAT	45.5	52.6	57.9	58.6	228 bp	1.0	56.6
trnR-UCU-trnfM-CAU_p3	AGAAGACCTCTGTCCTATCCA	CTTGGGGTTACGGGTTCGAT	47.6	55.0	56.9	59.7	223 bp	1.0	56.4
trnR-UCU-trnfM-CAU_p4	AGGTTTAGAAGACCTCTGTCCT	CTTGGGGTTACGGGTTCGAT	45.5	55.0	57.9	59.7	229 bp	1.0	55.6
trnR-UCU-trnfM-CAU_p5	AGAAGACCTCTGTCCTATCCA	TTGGGGTTACGGGTTCGA	47.6	55.6	56.9	58.1	222 bp	1.0	53.5