Hotspot

psbM-petN

LSC · 18491-19294 · 804 bp

Back to Arundinella

Pi 0.01

MarkerSeek score 61.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 22
pi: 0.01
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGTCAAAAGGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	55.7	58.3	837-864 bp	1.0	63.7
psbM-petN_p2	AAAACAGCCAGTCAAAAGGA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	55.7	58.4	836-863 bp	1.0	63.0
psbM-petN_p3	AAAACAGCCAGTCAAAAGGA	CGCTAGTAGTATGGGGGAGG	40.0	60.0	55.7	58.5	842-869 bp	1.0	62.3
psbM-petN_p4	AAAAACAGCCAGTCAAAAGGA	GTAGTATGGGGGAGGAGTGG	38.1	60.0	56.5	58.3	838-865 bp	1.0	62.0
psbM-petN_p5	AACAGCCAGTCAAAAGGATT	GTAGTATGGGGGAGGAGTGG	40.0	60.0	55.4	58.3	835-862 bp	1.0	61.8