Hotspot

trnG-UCC-trnM-CAU

LSC · 14117-16079 · 1963 bp

Back to Arundinella

Pi 0.0145

MarkerSeek score 58.10

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1963
variable_sites: 74
pi: 0.0145
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	GCATTACTCTGCCACTGAGT	38.1	50.0	55.5	57.6	2011-2031 bp	1.0	51.9
trnG-UCC-trnM-CAU_p2	AGAACGAATCACACTTTTACCAC	GCATTACTCTGCCACTGAGT	39.1	50.0	57.3	57.6	2014-2034 bp	1.0	50.9
trnG-UCC-trnM-CAU_p3	GAACGAATCACACTTTTACCACT	GCATTACTCTGCCACTGAGT	39.1	50.0	57.3	57.6	2013-2033 bp	1.0	50.9
trnG-UCC-trnM-CAU_p4	AACGAATCACACTTTTACCACT	GCATTACTCTGCCACTGAGT	36.4	50.0	56.2	57.6	2012-2032 bp	1.0	50.0
trnG-UCC-trnM-CAU_p5	ACGAATCACACTTTTACCACT	CTCGAACCGATGACTTCTGT	38.1	50.0	55.5	57.4	2107-2127 bp	1.0	49.8