Hotspot

rps16-trnQ-UUG

LSC · 5588-7074 · 1487 bp

Back to Arundinella

Pi 0.0113

MarkerSeek score 65.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1487
variable_sites: 44
pi: 0.0113
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1252-1577 bp	1.0	84.6
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1263-1588 bp	1.0	84.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1256-1581 bp	1.0	83.5
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1267-1592 bp	1.0	83.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AAACGAAATGAAATGGGGCG	45.0	45.0	58.1	57.6	1313-1638 bp	1.0	82.3