Hotspot

ycf4

LSC · 60163-60720 · 558 bp

Back to Arundinaria

Pi 0.00239

MarkerSeek score 56.70

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 558
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	CAATTTCAATCGACCGCTGC	TCCTCGTTTGTTCCTTTCTT	50.0	40.0	58.5	54.8	694-702 bp	1.0	51.1
ycf4_p2	AATTTCAATCGACCGCTGCT	TCCTCGTTTGTTCCTTTCTT	45.0	40.0	58.5	54.8	693-701 bp	1.0	50.4
ycf4_p3	GACCGCTGCTGGATTTAGTA	TCCTCGTTTGTTCCTTTCTT	50.0	40.0	57.4	54.8	683-691 bp	1.0	50.0
ycf4_p4	CAATTTCAATCGACCGCTGC	TCCTCGTTTGTTCCTTTCTTT	50.0	38.1	58.5	55.6	694-702 bp	1.0	49.7
ycf4_p5	CAATTTCAATCGACCGCTGC	ATCCTCGTTTGTTCCTTTCT	50.0	40.0	58.5	54.6	695-703 bp	1.0	49.2