Hotspot

rpoC2

LSC · 27163-31716 · 4554 bp

Back to Arundinaria

Pi 0.00264

MarkerSeek score 45.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4554
variable_sites: 18
pi: 0.00264
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GGGGCATTCTGATTTCGCTA	GAAAACTCTGTTGTTCCGCG	50.0	50.0	58.0	58.0	406 bp	1.0	82.3
rpoC2_p2	CTATTTGCGTACCGACCCAA	GAAAACTCTGTTGTTCCGCG	50.0	50.0	58.0	58.0	914 bp	1.0	81.1
rpoC2_p3	TATTTGCGTACCGACCCAAG	GAAAACTCTGTTGTTCCGCG	50.0	50.0	58.0	58.0	913 bp	1.0	81.1
rpoC2_p4	CTATTTGCGTACCGACCCAA	TGAGATGCATTTCCTCTGGC	50.0	50.0	58.0	58.0	1098 bp	1.0	80.7
rpoC2_p5	TATTTGCGTACCGACCCAAG	TGAGATGCATTTCCTCTGGC	50.0	50.0	58.0	58.0	1097 bp	1.0	80.7