Hotspot

atpF

LSC · 35254-36648 · 1395 bp

Back to Arundinaria

Pi 0.00956

MarkerSeek score 51.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1395
variable_sites: 20
pi: 0.00956
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGAGGATATGCTCGCCTTCT	CATCAACCCATCGCCCATTA	50.0	50.0	58.0	58.0	2001-2003 bp	1.0	80.9
atpF_p2	AGAGGATATGCTCGCCTTCT	TTTTGCCAGTCTGTCTGTCC	50.0	50.0	58.0	58.0	2284-2286 bp	1.0	80.8
atpF_p3	GTATTGCGAGACAGCCAGAA	CATCAACCCATCGCCCATTA	50.0	50.0	58.0	58.0	2319-2321 bp	1.0	80.7
atpF_p4	GTATTGCGAGACAGCCAGAA	TTTTGCCAGTCTGTCTGTCC	50.0	50.0	58.0	58.0	2602-2604 bp	1.0	80.6
atpF_p5	AGAGGATATGCTCGCCTTCT	CAATTTCGCCTCTCCCATCA	50.0	50.0	58.0	58.0	2123-2124 bp	0.666667	66.9