Hotspot

trnF-ndhJ

LSC · 50214-50796 · 583 bp

Back to Arundinaria

Pi 0.00458

MarkerSeek score 58.20

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 4
pi: 0.00458
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	692-705 bp	1.0	79.1
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	682-695 bp	1.0	78.5
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	723-736 bp	1.0	77.0
trnF-ndhJ_p4	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.5	57.9	695-708 bp	1.0	75.9
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	50.0	55.0	58.1	58.6	691-704 bp	1.0	75.3