Hotspot

psaA-ycf3

LSC · 43793-44418 · 626 bp

Back to Arundinaria

Pi 0.00747

MarkerSeek score 65.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 7
pi: 0.00747
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	726-735 bp	1.0	78.2
psaA-ycf3_p2	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	798-807 bp	1.0	78.1
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	722-731 bp	1.0	77.9
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	794-803 bp	1.0	77.8
psaA-ycf3_p5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.7	58.0	779-788 bp	1.0	76.7