Hotspot

petN-trnC

LSC · 19493-20402 · 910 bp

Back to Arundinaria

Pi 0.00147

MarkerSeek score 59.80

Primer available yes

Label petN-trnC

Feature Metadata

label_name: petN-trnC
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 2
pi: 0.00147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	958-959 bp	1.0	70.6
petN-trnC_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	960-961 bp	1.0	70.2
petN-trnC_p3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.3	58.1	1014-1015 bp	1.0	69.6
petN-trnC_p4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	60.0	55.0	58.3	59.1	1016-1017 bp	1.0	69.1
petN-trnC_p5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.4	58.1	1015-1016 bp	1.0	68.8