Hotspot

rps16-trnQ

LSC · 5606-7155 · 1550 bp

Back to Arundinaria

Pi 0.00173

MarkerSeek score 61.50

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1550
variable_sites: 4
pi: 0.00173
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1607-1624 bp	1.0	79.7
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1618-1635 bp	1.0	79.2
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1611-1628 bp	1.0	78.6
rps16-trnQ_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1622-1639 bp	1.0	78.1
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	TAAAACCAGAAATGGGGCGT	45.0	45.0	58.1	57.7	1667-1684 bp	1.0	77.9