Hotspot

rpl14-rpl16

LSC · 82083-82231 · 149 bp

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Pi 0.047

MarkerSeek score 79.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 7
pi: 0.047
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTATCCGCTACATT	TGAAATGAGCGGAGTAGCAG	50.0	50.0	58.1	57.7	290 bp	1.0	78.5
rpl14-rpl16_p2	TACACATCAATTCTCGGGCC	TGAAATGAGCGGAGTAGCAG	50.0	50.0	57.7	57.7	310 bp	1.0	76.8
rpl14-rpl16_p3	CCGCTGTTATCCGCTACATT	GAAATGAGCGGAGTAGCAGA	50.0	50.0	58.1	57.4	289 bp	1.0	76.2
rpl14-rpl16_p4	TACACATCAATTCTCGGGCC	GAAATGAGCGGAGTAGCAGA	50.0	50.0	57.7	57.4	309 bp	1.0	74.5
rpl14-rpl16_p5	TCCGCTACATTCAAATGGGT	TGAAATGAGCGGAGTAGCAG	45.0	50.0	57.2	57.7	281 bp	1.0	73.2