Hotspot

rpl36-rps8

LSC · 80637-81110 · 474 bp

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Pi 0.00633

MarkerSeek score 59.20

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 3
pi: 0.00633
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ACTATAATTCGTCCCCGCCT	ATAGAAGGAATCGGCGGAGA	50.0	50.0	58.3	58.0	590 bp	1.0	78.1
rpl36-rps8_p2	ACTATAATTCGTCCCCGCCT	GAAGGAATCGGCGGAGAAAT	50.0	50.0	58.3	58.0	587 bp	1.0	77.8
rpl36-rps8_p3	GCCTACGGATCAATCGACAT	ATAGAAGGAATCGGCGGAGA	50.0	50.0	57.6	58.0	574 bp	1.0	76.9
rpl36-rps8_p4	GCCTACGGATCAATCGACAT	GAAGGAATCGGCGGAGAAAT	50.0	50.0	57.6	58.0	571 bp	1.0	76.5
rpl36-rps8_p5	ACTATAATTCGTCCCCGCCT	TAGAAGGAATCGGCGGAGAA	50.0	50.0	58.3	58.2	589 bp	1.0	76.3