Hotspot

trnG-UCC-trnfM-CAU

LSC · 37677-37860 · 184 bp

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Pi 0.0163

MarkerSeek score 61.90

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 3
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	250 bp	1.0	80.2
trnG-UCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	251 bp	1.0	80.2
trnG-UCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	291 bp	1.0	79.7
trnG-UCC-trnfM-CAU_p4	AGAAGATGCGGGTTCGATTC	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.1	57.8	248 bp	1.0	79.1
trnG-UCC-trnfM-CAU_p5	GAGAAGATGCGGGTTCGATT	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.1	57.8	249 bp	1.0	79.1