Hotspot

petN-psbM

LSC · 29664-30885 · 1222 bp

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Pi 0.00409

MarkerSeek score 61.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 5
pi: 0.00409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	CGTAAAAACAGTTAGCCAAGGC	45.0	45.5	58.0	58.4	1326 bp	1.0	60.7
petN-psbM_p2	ATCTTGCTTGGGCTGCTTTA	CGTAAAAACAGTTAGCCAAGGC	45.0	45.5	57.8	58.4	1327 bp	1.0	59.0
petN-psbM_p3	TAAATCTTGCTTGGGCTGCT	CGTAAAAACAGTTAGCCAAGGC	45.0	45.5	57.8	58.4	1330 bp	1.0	59.0
petN-psbM_p4	GTAAATCTTGCTTGGGCTGC	CGTAAAAACAGTTAGCCAAGGC	50.0	45.5	57.7	58.4	1331 bp	1.0	58.5
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	ACGTAAAAACAGTTAGCCAAGG	45.0	40.9	58.0	57.2	1327 bp	1.0	57.7