Hotspot

rps16-trnQ-UUG

LSC · 6333-7211 · 879 bp

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Pi 0.00569

MarkerSeek score 54.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 5
pi: 0.00569
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	970-971 bp	1.0	79.6
rps16-trnQ-UUG_p2	GCGGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	968-969 bp	1.0	79.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	952-953 bp	1.0	78.7
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	963-964 bp	1.0	78.2
rps16-trnQ-UUG_p5	AGCGGATCATGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	969-970 bp	1.0	77.2