Hotspot

ycf1

SSC · 129809-135433 · 5625 bp

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Pi 0.0133

MarkerSeek score 42.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 168
pi: 0.0133
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCAAGGTTTCAGACGAAA	CGTACCCACCCAGTTTGTTA	45.0	50.0	58.0	57.5	1459-1540 bp	1.0	79.3
ycf1_p2	TGCCAAGGTTTCAGACGAAA	TTACGTACCCACCCAGTTTG	45.0	50.0	58.0	57.5	1462-1543 bp	1.0	79.3
ycf1_p3	TGCCAAGGTTTCAGACGAAA	TACGTACCCACCCAGTTTGT	45.0	50.0	58.0	58.6	1461-1542 bp	1.0	79.1
ycf1_p4	ATCCTCCGACCATTCAGGAA	CGTACCCACCCAGTTTGTTA	50.0	50.0	58.1	57.5	1319-1400 bp	1.0	79.0
ycf1_p5	ATCCTCCGACCATTCAGGAA	TTACGTACCCACCCAGTTTG	50.0	50.0	58.1	57.5	1322-1403 bp	1.0	79.0