Hotspot

ndhF

SSC · 114484-116760 · 2277 bp

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Pi 0.0123

MarkerSeek score 45.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2277
variable_sites: 65
pi: 0.0123
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TGGAGATCAGAGCCTTTCCT	50.0	50.0	58.0	57.7	2988-3000 bp	1.0	80.4
ndhF_p2	AAGAGGGATCCACCGAAGAA	TGGAGATCAGAGCCTTTCCT	50.0	50.0	58.0	57.7	2980-2992 bp	1.0	80.4
ndhF_p3	AGGGATCCACCGAAGAAGAT	TGGAGATCAGAGCCTTTCCT	50.0	50.0	57.8	57.7	2977-2989 bp	1.0	79.3
ndhF_p4	CAGGAACAAGAGGGATCCAC	TGGAGATCAGAGCCTTTCCT	55.0	50.0	57.6	57.7	2987-2999 bp	1.0	77.5
ndhF_p5	AGAGGGATCCACCGAAGAAG	TGGAGATCAGAGCCTTTCCT	55.0	50.0	58.5	57.7	2979-2991 bp	1.0	76.7