Hotspot

trnF-GAA-ndhJ

LSC · 51999-52670 · 672 bp

Back to Artocarpus

Pi 0.0109

MarkerSeek score 54.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 17
pi: 0.0109
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GAAAGCTGGATAGGATGGCC	55.0	55.0	58.0	58.1	811-819 bp	1.0	81.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.1	58.1	775-783 bp	1.0	81.3
trnF-GAA-ndhJ_p3	TGATGCGTAAGGAATGGTCG	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.1	58.1	830-838 bp	1.0	81.0
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	GAAAGCTGGATAGGATGGCC	55.0	55.0	57.9	58.1	799-807 bp	1.0	80.5
trnF-GAA-ndhJ_p5	AAGGAATGGTCGGGATAGCT	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.2	58.1	822-830 bp	1.0	80.0