Hotspot

ycf3-trnS-GGA

LSC · 47403-48128 · 726 bp

Back to Artocarpus

Pi 0.0145

MarkerSeek score 57.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 23
pi: 0.0145
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CTCTTTCTCGCCCGAAGTTG	AGGTGAAGGGTACGGAAAGA	55.0	50.0	58.9	58.0	916-928 bp	1.0	75.6
ycf3-trnS-GGA_p2	CTCTTTCTCGCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	55.0	50.0	58.9	58.1	884-896 bp	1.0	75.1
ycf3-trnS-GGA_p3	CTCTTTCTCGCCCGAAGTTG	ACGGAAAGAGAGGGATTCGA	55.0	50.0	58.9	58.2	905-917 bp	1.0	74.6
ycf3-trnS-GGA_p4	CTTTCTCGCCCGAAGTTGT	AGGTGAAGGGTACGGAAAGA	52.6	50.0	58.1	58.0	914-926 bp	1.0	74.4
ycf3-trnS-GGA_p5	TTTCTCGCCCGAAGTTGTC	AGGTGAAGGGTACGGAAAGA	52.6	50.0	58.1	58.0	913-925 bp	1.0	74.3