Hotspot

psbC-trnS-UGA

LSC · 37984-38236 · 253 bp

Back to Artocarpus

Pi 0.0281

MarkerSeek score 59.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 18
pi: 0.0281
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	403-414 bp	1.0	80.1
psbC-trnS-UGA_p2	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	402-413 bp	1.0	79.5
psbC-trnS-UGA_p3	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	437-448 bp	1.0	79.4
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	414-425 bp	1.0	79.4
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.4	58.1	448-459 bp	1.0	78.8