Hotspot

trnS-GCU-trnG-UCC

LSC · 9237-9945 · 709 bp

Back to Artocarpus

Pi 0.0163

MarkerSeek score 57.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 24
pi: 0.0163
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	770-802 bp	1.0	58.1
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	769-801 bp	1.0	58.1
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	773-805 bp	1.0	57.1
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	772-804 bp	1.0	57.1
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	771-803 bp	1.0	57.0