Hotspot

rpoB

LSC · 21603-24830 · 3228 bp

Back to Arthraxon

Pi 0.0018

MarkerSeek score 55.50

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3228
variable_sites: 14
pi: 0.0018
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TGCGAATCGGGCTTTAATGA	CTGCTCCCTCCGCTAAAATT	45.0	50.0	58.0	58.0	314 bp	1.0	80.2
rpoB_p2	GAACGCCAAACAGCTCTAGA	CTGCTCCCTCCGCTAAAATT	50.0	50.0	57.9	58.0	226 bp	1.0	80.1
rpoB_p3	TCGGTCTGAGAAGTGCATTG	CTGCTCCCTCCGCTAAAATT	50.0	50.0	57.9	58.0	260 bp	1.0	79.9
rpoB_p4	AAGCAGTTCCACTTTCTCGG	CTGCTCCCTCCGCTAAAATT	50.0	50.0	58.1	58.0	276 bp	1.0	79.6
rpoB_p5	TGCGAATCGGGCTTTAATGA	TTCCTAAAGCAAGTTCCCCG	45.0	50.0	58.0	57.8	344 bp	1.0	78.9