Hotspot

ndhK

LSC · 51349-52110 · 762 bp

Back to Arthraxon

Pi 0.0021

MarkerSeek score 56.00

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 762
variable_sites: 4
pi: 0.0021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TCTGCACAAAAGAACCCCTC	GGGCAATGAGTTTCGACGTA	50.0	50.0	58.0	58.3	876-891 bp	1.0	79.8
ndhK_p2	TCTGCACAAAAGAACCCCTC	ACCCTTGGGCAATGAGTTTC	50.0	50.0	58.0	58.4	882-897 bp	1.0	79.2
ndhK_p3	TCTGCACAAAAGAACCCCTC	CCTTGGGCAATGAGTTTCGA	50.0	50.0	58.0	58.5	880-895 bp	1.0	78.4
ndhK_p4	GATAACCAACCCTGCTGCAT	GGGCAATGAGTTTCGACGTA	50.0	50.0	58.2	58.3	974-989 bp	1.0	77.9
ndhK_p5	ATAACCAACCCTGCTGCATT	GGGCAATGAGTTTCGACGTA	45.0	50.0	57.8	58.3	973-988 bp	1.0	77.9